Nucleic Acid Electrophoresis:Article Title: Schistosoma Species and Hybrid Genotyping With a Field Deployable Multi‐Marker Amplicon Panel
Article Snippet: .. Whatman FTA cards (Sigma, WHAWB120205 or WHA10534612) or worms/eggs stored in RNA later stabilization solution (Thermo Fisher, AM7020) or 70% ethanol DNeasy Blood and Tissue Kit (Qiagen, 69506) containing: Buffer ATL Proteinase K Buffer AL Buffer AW1 concentrate Buffer AW2 concentrate Mini Spin columns 2‐ml collection tubes 100% ethanol H 2 O, Ambion nuclease‐free (Thermo Fisher, AM9937) PMIX 1 (see Strategic planning above) PMIX 2 (see Strategic planning above) 5× KAPA HiFi buffer (Roche, 07958846001): 10 mM KAPA dNTP mix 1 U/μl KAPA HiFi DNA ploymerase Gel electrophoresis reagents: UltraPure agarose powder (Thermo Fisher, 16500‐900) TAE/TBE (Apex Bioresearch, 20‐194) GelRed stain (VWR, 89139‐142) Bromophenol blue tracking dye (Quality Biological, 351‐028‐661) Unicore punch kit, 2.0‐mm (Qiagen, WB100029) 1.5‐ml Maxymum Recovery Snaplock microcentrifuge tubes (Axygen, MCT‐150‐L‐C) Pipettes (Gilson and Rainin brands) 10‐, 200‐, and 1000‐μl filtered pipette tips (PurePoint extra‐long, FT1010; PurePoint, FT1200; and CellTreat low retention extended length, 229022) Vortex mixer Incubator Eppendorf centrifuges 5810R and 5430R TempAssure 0.2‐ml PCR 8‐tube strips with individual caps attached (USA Scientific, 1402‐4700) Eppendorf Mastercycler nexus thermal cycler Gel electrophoresis equipment UV or blue‐light transilluminator Multichannel pipettes (Thermo Fisher, Finnpipette) .. Genomic DNA is extracted directly from Whatman FTA cards using the Qiagen DNeasy Blood and Tissue Kit following the manufacturer's instructions.
Staining:Article Title: Schistosoma Species and Hybrid Genotyping With a Field Deployable Multi‐Marker Amplicon Panel
Article Snippet: .. Whatman FTA cards (Sigma, WHAWB120205 or WHA10534612) or worms/eggs stored in RNA later stabilization solution (Thermo Fisher, AM7020) or 70% ethanol DNeasy Blood and Tissue Kit (Qiagen, 69506) containing: Buffer ATL Proteinase K Buffer AL Buffer AW1 concentrate Buffer AW2 concentrate Mini Spin columns 2‐ml collection tubes 100% ethanol H 2 O, Ambion nuclease‐free (Thermo Fisher, AM9937) PMIX 1 (see Strategic planning above) PMIX 2 (see Strategic planning above) 5× KAPA HiFi buffer (Roche, 07958846001): 10 mM KAPA dNTP mix 1 U/μl KAPA HiFi DNA ploymerase Gel electrophoresis reagents: UltraPure agarose powder (Thermo Fisher, 16500‐900) TAE/TBE (Apex Bioresearch, 20‐194) GelRed stain (VWR, 89139‐142) Bromophenol blue tracking dye (Quality Biological, 351‐028‐661) Unicore punch kit, 2.0‐mm (Qiagen, WB100029) 1.5‐ml Maxymum Recovery Snaplock microcentrifuge tubes (Axygen, MCT‐150‐L‐C) Pipettes (Gilson and Rainin brands) 10‐, 200‐, and 1000‐μl filtered pipette tips (PurePoint extra‐long, FT1010; PurePoint, FT1200; and CellTreat low retention extended length, 229022) Vortex mixer Incubator Eppendorf centrifuges 5810R and 5430R TempAssure 0.2‐ml PCR 8‐tube strips with individual caps attached (USA Scientific, 1402‐4700) Eppendorf Mastercycler nexus thermal cycler Gel electrophoresis equipment UV or blue‐light transilluminator Multichannel pipettes (Thermo Fisher, Finnpipette) .. Genomic DNA is extracted directly from Whatman FTA cards using the Qiagen DNeasy Blood and Tissue Kit following the manufacturer's instructions.
Transferring:Article Title: Schistosoma Species and Hybrid Genotyping With a Field Deployable Multi‐Marker Amplicon Panel
Article Snippet: .. Whatman FTA cards (Sigma, WHAWB120205 or WHA10534612) or worms/eggs stored in RNA later stabilization solution (Thermo Fisher, AM7020) or 70% ethanol DNeasy Blood and Tissue Kit (Qiagen, 69506) containing: Buffer ATL Proteinase K Buffer AL Buffer AW1 concentrate Buffer AW2 concentrate Mini Spin columns 2‐ml collection tubes 100% ethanol H 2 O, Ambion nuclease‐free (Thermo Fisher, AM9937) PMIX 1 (see Strategic planning above) PMIX 2 (see Strategic planning above) 5× KAPA HiFi buffer (Roche, 07958846001): 10 mM KAPA dNTP mix 1 U/μl KAPA HiFi DNA ploymerase Gel electrophoresis reagents: UltraPure agarose powder (Thermo Fisher, 16500‐900) TAE/TBE (Apex Bioresearch, 20‐194) GelRed stain (VWR, 89139‐142) Bromophenol blue tracking dye (Quality Biological, 351‐028‐661) Unicore punch kit, 2.0‐mm (Qiagen, WB100029) 1.5‐ml Maxymum Recovery Snaplock microcentrifuge tubes (Axygen, MCT‐150‐L‐C) Pipettes (Gilson and Rainin brands) 10‐, 200‐, and 1000‐μl filtered pipette tips (PurePoint extra‐long, FT1010; PurePoint, FT1200; and CellTreat low retention extended length, 229022) Vortex mixer Incubator Eppendorf centrifuges 5810R and 5430R TempAssure 0.2‐ml PCR 8‐tube strips with individual caps attached (USA Scientific, 1402‐4700) Eppendorf Mastercycler nexus thermal cycler Gel electrophoresis equipment UV or blue‐light transilluminator Multichannel pipettes (Thermo Fisher, Finnpipette) .. Genomic DNA is extracted directly from Whatman FTA cards using the Qiagen DNeasy Blood and Tissue Kit following the manufacturer's instructions.
Polymerase Chain Reaction:Article Title: Schistosoma Species and Hybrid Genotyping With a Field Deployable Multi‐Marker Amplicon Panel
Article Snippet: .. Whatman FTA cards (Sigma, WHAWB120205 or WHA10534612) or worms/eggs stored in RNA later stabilization solution (Thermo Fisher, AM7020) or 70% ethanol DNeasy Blood and Tissue Kit (Qiagen, 69506) containing: Buffer ATL Proteinase K Buffer AL Buffer AW1 concentrate Buffer AW2 concentrate Mini Spin columns 2‐ml collection tubes 100% ethanol H 2 O, Ambion nuclease‐free (Thermo Fisher, AM9937) PMIX 1 (see Strategic planning above) PMIX 2 (see Strategic planning above) 5× KAPA HiFi buffer (Roche, 07958846001): 10 mM KAPA dNTP mix 1 U/μl KAPA HiFi DNA ploymerase Gel electrophoresis reagents: UltraPure agarose powder (Thermo Fisher, 16500‐900) TAE/TBE (Apex Bioresearch, 20‐194) GelRed stain (VWR, 89139‐142) Bromophenol blue tracking dye (Quality Biological, 351‐028‐661) Unicore punch kit, 2.0‐mm (Qiagen, WB100029) 1.5‐ml Maxymum Recovery Snaplock microcentrifuge tubes (Axygen, MCT‐150‐L‐C) Pipettes (Gilson and Rainin brands) 10‐, 200‐, and 1000‐μl filtered pipette tips (PurePoint extra‐long, FT1010; PurePoint, FT1200; and CellTreat low retention extended length, 229022) Vortex mixer Incubator Eppendorf centrifuges 5810R and 5430R TempAssure 0.2‐ml PCR 8‐tube strips with individual caps attached (USA Scientific, 1402‐4700) Eppendorf Mastercycler nexus thermal cycler Gel electrophoresis equipment UV or blue‐light transilluminator Multichannel pipettes (Thermo Fisher, Finnpipette) .. Genomic DNA is extracted directly from Whatman FTA cards using the Qiagen DNeasy Blood and Tissue Kit following the manufacturer's instructions.
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